JOVE JOURNAL

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A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae

Muhammad Afzal^1,2, Irfan Manzoor^1,2, Oscar P. Kuipers¹

¹Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute,University of Groningen, ²Department of Bioinformatics and Biotechnology,Government College University Faisalabad

Journal (Biology)

A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids

Jooske F. Van Lidth de Jeude¹, Jacqueline L. M. Vermeulen¹, Paula S. Montenegro-Miranda¹, Gijs R. Van den Brink¹, Jarom Heijmans¹

¹Tytgat Institute for Liver and Intestinal Research, Department of Gasteroenterology and Hepatology,Academical Medical Center

Journal (Biology)

Determining Membrane Protein Topology Using Fluorescence Protease Protection (FPP)

Carl White¹, Alex Nixon¹, Neil A. Bradbury¹

¹Department of Physiology and Biophysics,Chicago Medical School

Journal (Biology)

A Proteoliposome-Based Efflux Assay to Determine Single-molecule Properties of Cl^– Channels and Transporters

Daniel Basilio¹, Alessio Accardi^1,2,3

¹Department of Anesthesiology,Weill Cornell Medical College, ²Department of Physiology and Biophysics,Weill Cornell Medical College, ³Department of Biochemistry,Weill Cornell Medical College

Journal (Biology)

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Wilson Wong¹, Ryan Farr¹, Mugdha Joglekar¹, Andrzej Januszewski², Anandwardhan Hardikar¹

¹Diabetes and Islet Biology Group, NHMRC Clinical Trials Centre, Faculty of Medicine,The University of Sydney, ²Biomarkers Laboratory, NHMRC Clinical Trials Centre, Faculty of Medicine,The University of Sydney

Journal (Biology)

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Cameron A. Smurthwaite¹, Wesley Williams¹, Alexandra Fetsko¹, Darin Abbadessa¹, Zachary D. Stolp¹, Connor W. Reed¹, Andre Dharmawan¹, Roland Wolkowicz¹

¹Department of Biology,San Diego State University

Journal (Biology)

RNA Isolation from Cell Specific Subpopulations Using Laser-capture Microdissection Combined with Rapid Immunolabeling

Audrey Chabrat*^1,2, Hélène Doucet-Beaupré*^1,2, Martin Lévesque^1,2

¹Department of Psychiatry and Neurosciences, Faculty of Medicine,Université Laval, ²Centre de recherche de l'Institut universitaire en santé mentale de Québec

Journal (Biology)

Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle

Jared M. Schrader¹, Lucy Shapiro¹

¹Department of Developmental Biology,Stanford University School of Medicine

Journal (Biology)

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays

Nydia Morales-Soto^1,2, Morgen E. Anyan¹, Anne E. Mattingly¹, Chinedu S. Madukoma¹, Cameron W. Harvey³, Mark Alber³, Eric Déziel⁴, Daniel B. Kearns⁵, Joshua D. Shrout^1,2,6

¹Department of Civil and Environmental Engineering and Earth Sciences,University of Notre Dame, ²Eck Institute for Global Health,University of Notre Dame, ³Department of Applied and Computational Mathematics and Statistics,University of Notre Dame, ⁴INRS-Institut Armand-Frappier, ⁵Department of Biology,Indiana University, ⁶Department of Biological Sciences,University of Notre Dame

Journal (Biology)

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae

David Dilworth¹, Christopher J. Nelson¹

¹Department of Biochemistry and Microbiology,University of Victoria

Journal (Biology)

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Zachary A. Crannell*¹, Brittany Rohrman*¹, Rebecca Richards-Kortum¹

¹Department of Bioengineering,Rice University

Journal (Biology)

Functional Characterization of Na⁺/H⁺ Exchangers of Intracellular Compartments Using Proton-killing Selection to Express Them at the Plasma Membrane

Nina Milosavljevic*¹, Mallorie Poët*¹, Michael Monet¹, Eléonore Birgy-Barelli¹, Isabelle Léna¹, Laurent Counillon¹

¹Université Nice-Sophia Antipolis, Laboratoire de Physiomédecine Moléculaire, CNRS UMR7370, and Laboratories of Excellence Ion Channel Science and Therapeutics

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