JOVE JOURNAL

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Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Qianfan Bai*¹, Zhiqiang Bai*¹, Shaohai Xu³, Lei Sun^1,2

¹Cardiovascular and Metabolic Disorders Program,Duke-NUS Medical School, ²Institute of Molecular and Cell Biology, Singapore, ³Division of Bioengineering, School of Chemical & Biomedical Engineering,Nanyang Technological University

Journal (Biology)

Optimal Lentivirus Production and Cell Culture Conditions Necessary to Successfully Transduce Primary Human Bronchial Epithelial Cells

Nathalie Baumlin-Schmid¹, Matthias Salathe¹, Nevis L. Fregien²

¹Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine,University of Miami, Miller School of Medicine, ²Department of Cell Biology,University of Miami, Miller School of Medicine

Journal (Biology)

Measurement of Survival Time in Brachionus Rotifers: Synchronization of Maternal Conditions

Gen Kaneko^1,4, Tatsuki Yoshinaga², Kristin E. Gribble³, David M. Welch³, Hideki Ushio¹

¹Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences,The University of Tokyo, ²School of Marine Biosciences, Department of Marine Biosciences,Kitasato University, ³Josephine Bay Paul Center for Comparative Molecular Biology and Evolution,Marine Biological Laboratory, ⁴School of Arts and Sciences,University of Houston-Victoria

Journal (Biology)

Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact

Justine Renaud¹, Maria-Grazia Martinoli¹

¹Dept. of Medical Biology,University of Québec

Journal (Biology)

Implementation of a Coherent Anti-Stokes Raman Scattering (CARS) System on a Ti:Sapphire and OPO Laser Based Standard Laser Scanning Microscope

Vasyl Mytskaniuk*¹, Fabrice Bardin*^2,3, Hassan Boukhaddaoui^1,5, Herve Rigneault⁴, Nicolas Tricaud¹

¹INSERM U1051, Institut des Neurosciences de Montpellier (INM), Université de Montpellier, ²Université de Nîmes, ³CNRS, IES, UMR 5214, ⁴Aix-Marseille Université, CNRS, École Centrale Marseille, Institut Fresnel, UMR 7249, ⁵Montpellier RIO Imaging (MRI)

Journal (Biology)

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury

Robert A. McKee^1,2, Rebecca A. Wingert^1,2

¹Center for Zebrafish Research, Department of Biological Sciences,University of Notre Dame, ²Center for Stem Cells and Regenerative Medicine, Department of Biological Sciences,University of Notre Dame

Journal (Biology)

Hydroponics: A Versatile System to Study Nutrient Allocation and Plant Responses to Nutrient Availability and Exposure to Toxic Elements

Nga T. Nguyen¹, Samuel A. McInturf¹, David G. Mendoza-Cózatl¹

¹Division of Plant Sciences, C.S. Bond Life Sciences Center,University of Missouri, Columbia

Journal (Biology)

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses

Agnieszka B. Bialkowska*¹, Amr M. Ghaleb*¹, Mandayam O. Nandan¹, Vincent W. Yang^1,2

¹Department of Medicine,Stony Brook University School of Medicine, ²Department of Physiology & Biophysics,Stony Brook University School of Medicine

Journal (Biology)

Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin

Kun-Wei Chan¹, Chieh Lo¹, Chi-Shih Chu², Li-Te Chin², Yu-Ting Wang¹, Wei-Cheng Yang¹

¹Department of Veterinary Medicine,National Chiayi University, ²Department of Microbiology and Immunology/Graduate Institute of Biomedical and Biopharmaceutical Sciences,National Chiayi University

Journal (Biology)

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes

Stefanie Arndt*¹, Heidi-Kristin Walter*¹, Hans-Achim Wagenknecht¹

¹Institute of Organic Chemistry,Karlsruhe Institute of Technology

Journal (Biology)

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing

Michael Allen Stiffler¹, Subu K Subramanian¹, Victor H Salinas¹, Rama Ranganathan¹

¹Green Center for Systems Biology,University of Texas Southwestern Medical Center

Journal (Biology)

Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside

Robyn A. Barbato¹, Natàlia Garcia-Reyero², Karen Foley¹, Robert Jones¹, Zoe Courville³, Thomas Douglas⁴, Edward Perkins², Charles M. Reynolds⁴

¹Biogeochemical Sciences Branch, Cold Regions Research and Engineering Laboratory,US Army Engineer Research & Development Center, Hanover, NH, ²Environmental Processes Branch, Environmental Laboratory,US Army Engineer Research & Development Center, Vicksburg, MS, ³Terrestrial and Cryospheric Scienes Branch, Cold Regions Research and Engineering Laboratory,US Army Engineer Research & Development Center, Hanover, NH, ⁴Biogeochemical Sciences Branch, Cold Regions Research and Engineering Laboratory,US Army Engineer Research & Development Center, Fairbanks, AK

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