JOVE JOURNAL

생화학

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Quantification of Subcellular Glycogen Distribution in Skeletal Muscle Fibers using Transmission Electron Microscopy

Rasmus Jensen¹, Niels Ørtenblad², Cristiano di Benedetto³, Klaus Qvortrup³, Joachim Nielsen²

¹Research center for applied health science,University College South Denmark, ²Department of Sports Science and Clinical Biomechanics,University of Southern Denmark, ³Department of Biomedical Sciences, Core Facility for Integrated Microscopy,University of Copenhagen

Journal (Biochemistry)

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Hoang P. M. Nguyen*¹, Kelly L. McGuire*¹, Brian D. Cook*¹, Mark A. Herzik, Jr.¹

¹Department of Chemistry and Biochemistry,University of California, San Diego

Journal (Biochemistry)

Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells

Himanshu Pandey¹, Tatiana Zvagelsky¹, Mary Popov¹, Mayan Sadan¹, Neta Yanir¹, Alina Goldstein-Levitin¹, Nurit Siegler¹, Shira Hershfinkel¹, Yahel Abraham¹, Roy Avraham¹, Levi A. Gheber², Larisa Gheber¹

¹Department of Chemistry,Ben-Gurion University of the Negev, Israel, ²Department of Biotechnology Engineering,Ben-Gurion University of the Negev, Israel

Journal (Biochemistry)

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

Megan C. DiIorio¹, Arkadiusz W. Kulczyk^1,2

¹Institute for Quantitative Biomedicine,Rutgers University, ²Department of Biochemistry & Microbiology,Rutgers University

Journal (Biochemistry)

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Jordan Demone¹, Kiah A. Barton^1,2, Illimar Altosaar^1,2

¹Department of Biochemistry, Microbiology, and Immunology,University of Ottawa, ²Proteins Easy Corp

Journal (Biochemistry)

Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms

Alexandre Dias Tavares Costa^1,2,3, Steffanie Skau Amadei^1,3, Amanda Bertão-Santos^1,2, Tuany Rodrigues^1,3

¹Laboratório de Ciências e Tecnologias Aplicadas em Saúde (LaCTAS), Instituto Carlos Chagas (ICC),Fundação Oswaldo Cruz (Fiocruz), ²Pós-graduação em Engenharia de Bioprocessos e Biotecnologia,Universidade Federal do Paraná (UFPR), ³Pós-graduação em Biociências e Biotecnologia, Instituto Carlos Chagas (ICC),Fundação Oswaldo Cruz (Fiocruz)

Journal (Biochemistry)

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Zekiye Ceren Arituluk^1,2, Bishnu Adhikari¹, Urmila Maitra¹, Caroline Goodman¹, Lukasz M. Ciesla¹

¹Department of Biological Sciences,The University of Alabama, ²Department of Pharmaceutical Botany, Faculty of Pharmacy,Hacettepe University

Journal (Biochemistry)

Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth

Mika Gallati¹, Bryant Point¹, Christopher W. Reid^1,2

¹Department of Science and Technology,Bryant University, ²Center for Health and Behavioral Sciences,Bryant University

Journal (Biochemistry)

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria

Ashley N. Oliveira¹, Brandon J. Richards¹, David A. Hood¹

¹Muscle Health Research Center, School of Kinesiology and Health Science,York University

Journal (Biochemistry)

Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates

Siyu Guo¹, Zhao Liu¹, Yongshuai Yang¹, Jun Chen¹, Chun Loong Ho¹

¹Department of Biomedical Engineering,Southern University of Science and Technology

Journal (Biochemistry)

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays

Ruchir C. Bobde*^1,2, Ketul Saharan*^1,2, Somanath Baral^1,3, Surajit Gandhi^1,2, Archana Samal^1,2, Rajivgandhi Sundaram¹, Ashish Kumar^1,4, Ajit K. Singh^1,5, Aritreyee Datta¹, Dileep Vasudevan¹

¹Institute of Life Sciences, ²Regional Centre for Biotechnology, ³School of Biotechnology,KIIT University, ⁴Department of Molecular Biophysics and Biochemistry,Yale University, ⁵Department of Pharmacology,University of Vermont College of Medicine

Journal (Biochemistry)

Purification of Endogenous Drosophila Transient Receptor Potential Channels

Jia Liu*¹, Yuyang Liu*¹, Weidi Chen¹, Yuzhen Ding¹, Xiaoru Lan¹, Wei Liu¹

¹Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute,Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center

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