JOVE JOURNAL

생화학

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Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

Hui-Lan Chang*¹, Kang-Yi Su*^1,2, Steven D. Goodman, Wern-Cherng Cheng, Liang-In Lin², Ya-Chien Yang², Sui-Yuan Chang², Woei-horng Fang²

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine,National Taiwan University, ²Department of Laboratory Medicine,National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children’s Hospital and the Department of Pediatrics,The Ohio State University

Journal (Biochemistry)

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

Shawn W. Schowe, Conner J. Langeberg, Erich G. Chapman, Kitty Brown, Marino J. E. Resendiz

¹Department of Chemistry,University of Colorado, Denver, ²Department of Chemistry & Biochemistry,University of Denver, ³Analytical Resources Core Bioanalysis & Omics,Colorado State University

Journal (Biochemistry)

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Matthew Payea, Myriam Gorospe, Nathan Basisty

¹Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program,National Institutes of Health, ²Translational Gerontology Branch, National Institute on Aging Intramural Research Program,National Institutes of Health

Journal (Biochemistry)

Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo

Shashi Bhushan Kumar, Shanvanth Arnipalli, Adham Abushukur, Silvia Carrau, Priyanka Mehta, Ouliana Ziouzenkova

¹Department of Human Sciences,The Ohio State University

Journal (Biochemistry)

Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

Nadezhda Podoplelova², Polina Soloveva⁴, Andrei Garzon Dasgupta^2,3, Aleksandra Filkova², Mikhail Panteleev^2,3,4

¹Center for Theoretical Problems of Physicochemical Pharmacology,Russian Academy of Sciences, ²National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, ³Faculty of Physics,Lomonosov Moscow State University, ⁴Faculty of Biological and Medical Physics,Moscow Institute of Physics and Technology

Journal (Biochemistry)

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

Sohee Choi, Jessica A. San Juan, Marie C. Heffern, Michael J. Stevenson

¹Department of Chemistry,University of San Francisco, ²Department of Chemistry,University of California, Davis

Journal (Biochemistry)

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Yana Lerner*¹, Surya Sukumaran*¹, Mei-Sze Chua, Samuel K. So, Nir Qvit

¹The Azrieli Faculty of Medicine in the Galilee,Bar-Ilan University, ²Asian Liver Center, Department of Surgery,Stanford University School of Medicine

Journal (Biochemistry)

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method

Léa Fermont, Nicolas Szydlowski², Christophe Colleoni

¹CNRS, UMR8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle,University of Lille, ²CNRS, USR3290-MSAP-Miniaturisation pour la Synthèse, l’Analyse et la Protéomique,University of Lille

Journal (Biochemistry)

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography

Alexander J. Bolt*¹, Linh D. Do*¹, Maryam Raeeszadeh-Sarmazdeh

¹Department of Chemical & Materials Engineering,University of Nevada, Reno

Journal (Biochemistry)

Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays

Santos Ramírez-Carreto, Sandra I. Salazar-García, Giovanni Macías Martínez, Claudia Rodríguez-Almazán²

¹Instituto de Biotecnología,Universidad Nacional Autónoma de México, ²Departamento de Micro y Nanotecnologías,Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México

Journal (Biochemistry)

Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

Roberto Angelini*¹, Silvia Russo*², Angela Corcelli, Simona Lobasso

¹Medical School,Swansea University, ²Department of Basic Medical Sciences, Neuroscience and Sense Organs,University of Bari Aldo Moro

Journal (Biochemistry)

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

Masatoshi Maeki², Yuto Okada, Shuya Uno, Ayuka Niwa, Akihiko Ishida, Hirofumi Tani, Manabu Tokeshi

¹Division of Applied Chemistry, Faculty of Engineering,Hokkaido University, ²JST PRESTO, ³Graduate School of Chemical Sciences and Engineering,Hokkaido University

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