JOVE JOURNAL

생화학

Showing 73 - 84 in 776 videos.

1…6 7 8 9 10 11…65

Journal (Biochemistry)

The Development and Application of Biophysical Assays for Evaluating Ternary Complex Formation Induced by Proteolysis Targeting Chimeras (PROTACS)

Wei Jiang¹, Holly Soutter¹

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

Isothermal Titration Calorimetry for Evaluating PROTACS-Induced Ternary Complex Formation

Wei Jiang¹, Holly Soutter¹

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

Biolayer Interferometry (BLI) for Evaluating PROTACS-Induced Ternary Complex Formation

Wei Jiang¹, Holly Soutter¹

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

Surface Plasmon Resonance (SPR) for Evaluating PROTACS-Induced Ternary Complex Formation

Wei Jiang¹, Holly Soutter¹

¹Center for the Development of Therapeutics,The Broad Institute of MIT and Harvard

Journal (Biochemistry)

Designing Streptavidin Affinity Grids for Cryo-Electron Microscopy

Trinity Cookis¹, Paul Sauer^2,3, Simon Poepsel^4,5, Bong-Gyoon Han⁶, Dominik A. Herbst^1,2,6, Robert Glaeser⁶, Eva Nogales^1,2,3,6

¹Department of Molecular and Cell Biology,University of California, Berkeley, ²California Institute for Quantitative Biology (QB3),University of California, Berkeley, ³Howard Hughes Medical Institute,University of California, Berkeley, ⁴Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital,University of Cologne, ⁵Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD),University of Cologne, ⁶Molecular Biophysics and Integrative Bio-Imaging Division,Lawrence Berkeley National Laboratory

Journal (Biochemistry)

Streptavidin-Affinity Grid Fabrication for Cryo-Electron Microscopy Sample Preparation

Trinity Cookis¹, Paul Sauer^2,3, Simon Poepsel^4,5, Bong-Gyoon Han⁶, Dominik A. Herbst^1,2,6, Robert Glaeser⁶, Eva Nogales^1,2,3,6

¹Department of Molecular and Cell Biology,University of California, Berkeley, ²California Institute for Quantitative Biology (QB3),University of California, Berkeley, ³Howard Hughes Medical Institute,University of California, Berkeley, ⁴Center for Molecular Medicine Cologne (CMMC), Faculty of Medicine and University Hospital,University of Cologne, ⁵Cologne Excellence Cluster for Cellular Stress Responses in Ageing-Associated Diseases (CECAD),University of Cologne, ⁶Molecular Biophysics and Integrative Bio-Imaging Division,Lawrence Berkeley National Laboratory

Journal (Biochemistry)

Preparing Electron Microscopy (EM) Grids for Cellular Cryotomography

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen², Thomas Lidahl^2,3, Luiza Mendonça²

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Cell Seeding and Transfection on Electron Microscopy (EM) Grids for Cryotomography

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen², Thomas Lidahl^2,3, Luiza Mendonça²

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Plunge Freezing of Electron Microscopy (EM) Grids for Cryotomography

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen², Thomas Lidahl^2,3, Luiza Mendonça²

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Sample Preparation for In Situ Cryotomography of Mammalian Cells

Noah Weber*^1,2, Brennan Hinks*^2,3, Jacob Jensen², Thomas Lidahl^2,3, Luiza Mendonça²

¹College of Liberal Arts,University of Minnesota, ²Department of Biochemistry, Molecular Biology and Biophysics, Medical School,University of Minnesota, ³College of Biological Sciences,University of Minnesota

Journal (Biochemistry)

Using Smart Leginon Autoscreen for CryoEM Grid Screening

Anjelique Sawh-Gopal^1,2, Aygul Ishemgulova*¹, Eugene Y. D. Chua*¹, Mahira F. Aragon*¹, Joshua H. Mendez¹, Edward T. Eng¹, Alex J. Noble¹

¹Simons Electron Microscopy Center, New York Structural Biology Center, ²Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology

Journal (Biochemistry)

Cryo-Electron Microscopy Screening Automation Across Multiple Grids Using Smart Leginon

Anjelique Sawh-Gopal^1,2, Aygul Ishemgulova*¹, Eugene Y. D. Chua*¹, Mahira F. Aragon*¹, Joshua H. Mendez¹, Edward T. Eng¹, Alex J. Noble¹

¹Simons Electron Microscopy Center, New York Structural Biology Center, ²Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology

1…6 7 8 9 10 11…65