JOVE JOURNAL

생화학

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Journal (Biochemistry)

Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion

Sarah Wazir¹, Danny Farhat¹, Mahalashmi Srinivasan^1,2, Jyh-Yeuan Lee¹

¹Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine,University of Ottawa, ²Department of Biochemistry, Faculty of Medicine and Dentistry,University of Alberta

Journal (Biochemistry)

ABCG5/G8 Crystallization in a Lipidic Bicelle Environment for X-Ray Crystallography

Sarah Wazir¹, Danny Farhat¹, Mahalashmi Srinivasan^1,2, Jyh-Yeuan Lee¹

¹Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine,University of Ottawa, ²Department of Biochemistry, Faculty of Medicine and Dentistry,University of Alberta

Journal (Biochemistry)

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

Stephanie Stransky¹, Dejauwne Young¹, Karoline Mikkelsen^2,3, Annemette Præstegaard Thulesen², Helle Sedighi Frandsen³, Simone Sidoli¹

¹Department of Biochemistry,Albert Einstein College of Medicine, ²Department of Biochemistry and Molecular Biology,University of Southern Denmark, ³CelVivo ApS

Journal (Biochemistry)

A Comprehensive Approach to Analyze the Cell Components of Cerebral Blood Clots

Wenwu Lin*¹, Hongjun Lin*², Pengliang Xin³, Hui Yan², Bin Kang⁴, Mingqing Tang²

¹Emergency Department,Quanzhou First Hospital Affiliated to Fujian Medical University, ²Medicine School,Huaqiao University, ³Department of Haematology,Quanzhou First Hospital Affiliated to Fujian Medical University, ⁴Department of Laboratory Medicine,Quanzhou First Hospital Affiliated to Fujian Medical University

Journal (Biochemistry)

Cerebral Thrombus Clot Dissolution and Cell Staining for Cell Component Analyses

Wenwu Lin*¹, Hongjun Lin*², Pengliang Xin³, Hui Yan², Bin Kang⁴, Mingqing Tang²

¹Emergency Department,Quanzhou First Hospital Affiliated to Fujian Medical University, ²Medicine School,Huaqiao University, ³Department of Haematology,Quanzhou First Hospital Affiliated to Fujian Medical University, ⁴Department of Laboratory Medicine,Quanzhou First Hospital Affiliated to Fujian Medical University

Journal (Biochemistry)

Isolating Small Extracellular Vesicles (sEVs) by Differential Ultracentrifugation

Jiale Cheng^1,2, Jiong Zhu^1,2, Yujiao Liu^2,3, Chen Yang^2,3, Yuehui Zhang², Yuchen Liu², Chaozhi Jin², Jian Wang^1,2

¹School of Basic Medical Sciences,Anhui Medical University, ²State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing),Beijing Institute of Lifeomics, ³School of Life Sciences,Hebei University

Journal (Biochemistry)

Extraction of Peptides from Isolated Small Extracellular Vesicles (sEVs)

Jiale Cheng^1,2, Jiong Zhu^1,2, Yujiao Liu^2,3, Chen Yang^2,3, Yuehui Zhang², Yuchen Liu², Chaozhi Jin², Jian Wang^1,2

¹School of Basic Medical Sciences,Anhui Medical University, ²State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing),Beijing Institute of Lifeomics, ³School of Life Sciences,Hebei University

Journal (Biochemistry)

Identification of Peptides of Small Extracellular Vesicles from Bone Marrow-Derived Macrophages

Jiale Cheng^1,2, Jiong Zhu^1,2, Yujiao Liu^2,3, Chen Yang^2,3, Yuehui Zhang², Yuchen Liu², Chaozhi Jin², Jian Wang^1,2

¹School of Basic Medical Sciences,Anhui Medical University, ²State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing),Beijing Institute of Lifeomics, ³School of Life Sciences,Hebei University

Journal (Biochemistry)

Preparation of Hard Palm Seeds for Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry Analysis

Gabriel R. Martins*^1,2, Felipe L. Brum*^1,2,3, Davi Marconi Miranda Carvalho da Silva*^1,2, Livia C. Barbosa³, Ronaldo Mohana-Borges³, Ayla Sant'Ana da Silva^1,2

¹Laboratório de Biocatálise (LABIC),Instituto Nacional de Tecnologia, ²Departamento de Bioquímica, Instituto de Química,Universidade Federal do Rio de Janeiro, ³Centro de Espectrometria de Massas de Biomoléculas, Instituto de Biofísica Carlos Chagas Filho,Universidade Federal do Rio de Janeiro

Journal (Biochemistry)

Seed Sectioning Protocol and Matrix Deposition Technique for MALDI-IMS Analysis of Hard Palm Seeds

Gabriel R. Martins*^1,2, Felipe L. Brum*^1,2,3, Davi Marconi Miranda Carvalho da Silva*^1,2, Livia C. Barbosa³, Ronaldo Mohana-Borges³, Ayla Sant'Ana da Silva^1,2

¹Laboratório de Biocatálise (LABIC),Instituto Nacional de Tecnologia, ²Departamento de Bioquímica, Instituto de Química,Universidade Federal do Rio de Janeiro, ³Centro de Espectrometria de Massas de Biomoléculas, Instituto de Biofísica Carlos Chagas Filho,Universidade Federal do Rio de Janeiro

Journal (Biochemistry)

Image Acquisition and MALDI-IMS Analysis of Hard Palm Seeds

Gabriel R. Martins*^1,2, Felipe L. Brum*^1,2,3, Davi Marconi Miranda Carvalho da Silva*^1,2, Livia C. Barbosa³, Ronaldo Mohana-Borges³, Ayla Sant'Ana da Silva^1,2

¹Laboratório de Biocatálise (LABIC),Instituto Nacional de Tecnologia, ²Departamento de Bioquímica, Instituto de Química,Universidade Federal do Rio de Janeiro, ³Centro de Espectrometria de Massas de Biomoléculas, Instituto de Biofísica Carlos Chagas Filho,Universidade Federal do Rio de Janeiro

Journal (Biochemistry)

Improvement of Bacillus subtilis Spore Enumeration and Label Analysis in Flow Cytometry

Késsia Caroline Alves^1,2, Yury Oliveira Chaves^1,3,4, Maria Edilene Almeida^1,4, Maria Gabriella Vasconcelos^1,4, Paulo Afonso Nogueira^1,3,6, Joyce Melo^1,9, Jéssica Marques⁸, Juliana Pavan Zuliani⁷, Charles Nunes Boeno⁷, Mauro Valentino Paloschi⁷, Rachele Isticato⁵, Ezio Ricca⁵, Luís André Mariúba^1,2,3,6

¹Laboratório de diagnóstico e controle de doenças infecciosas na Amazônia,Instituto Leônidas e Maria Deane - Fiocruz Amazônia, ²Programa de Pós-Graduação em Biotecnologia, Instituto de Ciências Biológicas,Universidade Federal do Amazonas (UFAM), ³Programa de Pós-Graduação em Biologia da Interação Patógeno-Hospedeiro - Fiocruz Amazônia, ⁴Programa de Pós-graduação em Ciências Aplicadas à Hematologia PPGH-UEA/HEMOAM, ⁵Department of Biology,Federico II University, ⁶Programa de Pós-Graduação em Imunologia Básica e Aplicada, Instituto de Ciências Biológicas,Universidade Federal do Amazonas (UFAM), ⁷Laboratório de Imunologia Celular Aplicada à saúde, Fundação Oswaldo Cruz,FIOCRUZ Rondônia, ⁸Centro Multiusuário para Análises de Fenômenos Biomédicos (CMABio-UEA),Universidade Estadual do Amazonas, ⁹Universidade Federal do Amazonas (UFAM)

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