JOVE JOURNAL

생화학

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Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Matthew Payea¹, Myriam Gorospe¹, Nathan Basisty²

¹Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program,National Institutes of Health, ²Translational Gerontology Branch, National Institute on Aging Intramural Research Program,National Institutes of Health

Journal (Biochemistry)

Extracellular Glucose Depletion as an Indirect Measure of Glucose Uptake in Cells and Tissues Ex Vivo

Shashi Bhushan Kumar¹, Shanvanth Arnipalli¹, Adham Abushukur¹, Silvia Carrau¹, Priyanka Mehta¹, Ouliana Ziouzenkova¹

¹Department of Human Sciences,The Ohio State University

Journal (Biochemistry)

Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

Nadezhda Podoplelova^1,2, Polina Soloveva^1,4, Andrei Garzon Dasgupta^1,2,3, Aleksandra Filkova^1,2, Mikhail Panteleev^1,2,3,4

¹Center for Theoretical Problems of Physicochemical Pharmacology,Russian Academy of Sciences, ²National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, ³Faculty of Physics,Lomonosov Moscow State University, ⁴Faculty of Biological and Medical Physics,Moscow Institute of Physics and Technology

Journal (Biochemistry)

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

Sohee Choi¹, Jessica A. San Juan², Marie C. Heffern², Michael J. Stevenson¹

¹Department of Chemistry,University of San Francisco, ²Department of Chemistry,University of California, Davis

Journal (Biochemistry)

Exploring Biomolecular Interaction Between the Molecular Chaperone Hsp90 and Its Client Protein Kinase Cdc37 using Field-Effect Biosensing Technology

Yana Lerner*¹, Surya Sukumaran*¹, Mei-Sze Chua², Samuel K. So², Nir Qvit¹

¹The Azrieli Faculty of Medicine in the Galilee,Bar-Ilan University, ²Asian Liver Center, Department of Surgery,Stanford University School of Medicine

Journal (Biochemistry)

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis (FACE) Method

Léa Fermont¹, Nicolas Szydlowski^1,2, Christophe Colleoni¹

¹CNRS, UMR8576-UGSF-Unité de Glycobiologie Structurale et Fonctionnelle,University of Lille, ²CNRS, USR3290-MSAP-Miniaturisation pour la Synthèse, l’Analyse et la Protéomique,University of Lille

Journal (Biochemistry)

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography

Alexander J. Bolt*¹, Linh D. Do*¹, Maryam Raeeszadeh-Sarmazdeh¹

¹Department of Chemical & Materials Engineering,University of Nevada, Reno

Journal (Biochemistry)

Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays

Santos Ramírez-Carreto¹, Sandra I. Salazar-García¹, Giovanni Macías Martínez¹, Claudia Rodríguez-Almazán^1,2

¹Instituto de Biotecnología,Universidad Nacional Autónoma de México, ²Departamento de Micro y Nanotecnologías,Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México

Journal (Biochemistry)

Fingerprinting Cardiolipin in Leukocytes by Mass Spectrometry for a Rapid Diagnosis of Barth Syndrome

Roberto Angelini*¹, Silvia Russo*², Angela Corcelli², Simona Lobasso²

¹Medical School,Swansea University, ²Department of Basic Medical Sciences, Neuroscience and Sense Organs,University of Bari Aldo Moro

Journal (Biochemistry)

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

Masatoshi Maeki^1,2, Yuto Okada³, Shuya Uno³, Ayuka Niwa³, Akihiko Ishida¹, Hirofumi Tani¹, Manabu Tokeshi¹

¹Division of Applied Chemistry, Faculty of Engineering,Hokkaido University, ²JST PRESTO, ³Graduate School of Chemical Sciences and Engineering,Hokkaido University

Journal (Biochemistry)

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Di Wu¹, Mengfan He¹, Dawei Li¹

¹Center for Translational Medicine,The Affiliated Zhangjiagang Hospital of Soochow University

Journal (Biochemistry)

Quantification of Humic and Fulvic Acids in Humate Ores, DOC, Humified Materials and Humic Substance-Containing Commercial Products

Richard T. Lamar¹, Hiarhi Monda¹

¹Bio Huma Netics, Inc.

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