Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Daniel Ram&#237;rez Montero; Humberto S&#225;nchez; Edo van Veen; Theo van Laar; Bel&#233;n Solano; John F. X. Diffley; Nynke H. Dekker

doi:10.3791/67076

Journal

생화학

Imaging the Motion of Fully Reconstituted Cdc45/Mcm2-7/GINS (CMG)

July 26, 2024

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

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Daniel Ramírez Montero¹, Humberto Sánchez¹, Edo van Veen¹, Theo van Laar¹, Belén Solano¹, John F. X. Diffley², Nynke H. Dekker^1,3

¹Department of Bionanoscience, Kavli Institute of Nanoscience,Delft University of Technology, ²Chromosome Replication Laboratory,Francis Crick Institute, ³Department of Physics and Kavli Institute of Nanoscience Discovery,University of Oxford

Summary

We report a hybrid ensemble and single-molecule assay to directly image and quantify the motion of fluorescently labeled, fully reconstituted Cdc45/Mcm2-7/GINS (CMG) helicase on linear DNA molecules held in place in an optical trap.

Imaging the Motion of Fully Reconstituted Cdc45/Mcm2-7/GINS (CMG)

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Summary

Article