1Carbohydrates and Enzymology Laboratory (CHEM-LAB), Department of Plant Sciences,University of the Free State, 2Faculty of Natural and Agricultural Sciences, Department of Plant Science, Botany, Plant Biochemistry and Physiology,University of the Free State, 3Agricultural Research Council – Small Grain Institute
The present protocol describes procedures used to study and characterize cell wall-related enzymes, mainly β-1,3-glucanase and peroxidase, in wheat plants. Their activity levels increase during wheat-RWA interaction and are involved in the plant defense response through cell wall reinforcement, which deters aphid feeding.
