1Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China,the Second Affiliated Hospital, Guangzhou Medical University, 2The Second Clinical Medicine School of Guangzhou Medical University, 3Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases,The Third Affiliated Hospital of Guangzhou Medical University, 4Key Laboratory for Reproductive Medicine of Guangdong Province,The Third Affiliated Hospital of Guangzhou Medical University
Nous présentons ici un protocole d’imagerie calcique ex vivo chez la drosophile adulte exprimant GCaMP6 pour surveiller les activités épileptiformes. Le protocole fournit un outil précieux pour étudier les événements ictaux chez la drosophile adulte par imagerie calcique ex vivo , permettant d’explorer les mécanismes potentiels de l’épilepsie au niveau cellulaire.
