Uma Lakshmipathy Life Sciences Groups Thermo Fisher Scientific Biography Publications Institution JoVE Articles Uma LakshmipathyDirector R&D Uma Lakshmipathy, is the Director of R&D in Cell Biology at Thermo Fisher Scientific. She received her doctoral degree in Molecular Biophysics from the Center for Cellular and Molecular Biology in India and postdoctoral experience in DNA double strand break repair from University of Minnesota Medical School. As a junior faculty at the Stem Cell Institute, University of Minnesota, her work involved developing ex vivo gene repair systems that enable correction of single gene mutations in adult stem cells. Her current work is focused on the development of novel products and services related to stem cells. Her work has resulted in the development of novel cell engineering platform, robust somatic reprogramming systems and comprehensive characterization tools. She is currently involved in clinical-grade pluripotent stem cell generation and cell therapy characterization platforms. Publications Generation and Comprehensive Characterization of Induced Pluripotent Stem Cells for Translational Research Regenerative Medicine. 06, 2019 | Pubmed ID: 31115261 Good Cell Culture Practice for Stem Cells and Stem-cell-derived Models ALTEX. Month, 2017 | Pubmed ID: 27554434 Characterizing Pluripotent Stem Cells Using the TaqMan® HPSC Scorecard(TM) Panel Methods in Molecular Biology (Clifton, N.J.). Month, 2016 | Pubmed ID: 25138722 Current Methods and Challenges in the Comprehensive Characterization of Human Pluripotent Stem Cells Stem Cell Reviews. Apr, 2015 | Pubmed ID: 25504379 CD44 is a Negative Cell Surface Marker for Pluripotent Stem Cell Identification During Human Fibroblast Reprogramming PloS One. Month, 2014 | Pubmed ID: 24416407 Advances in Genetic Modification of Pluripotent Stem Cells Biotechnology Advances. Nov, 2013 | Pubmed ID: 23856320 Stable Transfection Using Episomal Vectors to Create Modified Human Embryonic Stem Cells Methods in Molecular Biology (Clifton, N.J.). Month, 2013 | Pubmed ID: 23546763 Development and Characterization of a Clinically Compliant Xeno-free Culture Medium in Good Manufacturing Practice for Human Multipotent Mesenchymal Stem Cells Stem Cells Translational Medicine. Oct, 2012 | Pubmed ID: 23197667 Novel Live Alkaline Phosphatase Substrate for Identification of Pluripotent Stem Cells Stem Cell Reviews. Sep, 2012 | Pubmed ID: 22426885 Chromatin Insulator Elements Block Transgene Silencing in Engineered Human Embryonic Stem Cell Lines at a Defined Chromosome 13 Locus Stem Cells and Development. Jan, 2012 | Pubmed ID: 21699412 Efficient Non-viral Integration and Stable Gene Expression in Multipotent Adult Progenitor Cells Stem Cells International. Month, 2011 | Pubmed ID: 21977042 BacMam-mediated Gene Delivery into Multipotent Mesenchymal Stromal Cells Methods in Molecular Biology (Clifton, N.J.). Month, 2011 | Pubmed ID: 21431539 MiRNA in Pluripotent Stem Cells Regenerative Medicine. Jul, 2010 | Pubmed ID: 20632858 A Novel Serum-free Medium for the Expansion of Human Mesenchymal Stem Cells Stem Cell Research & Therapy. Month, 2010 | Pubmed ID: 20504289 HESC Engineering by Integrase-mediated Chromosomal Targeting Methods in Molecular Biology (Clifton, N.J.). Month, 2010 | Pubmed ID: 19907981 Global Characterization and Genomic Stability of Human MultiStem, A Multipotent Adult Progenitor Cell Journal of Stem Cells. Month, 2009 | Pubmed ID: 20498688 A Simplified Culture and Polymerase Chain Reaction Identification Assay for Quality Control Performance Testing of Stem Cell Media Products Cytotherapy. Month, 2009 | Pubmed ID: 19878062 Generation of Platform Human Embryonic Stem Cell Lines That Allow Efficient Targeting at a Predetermined Genomic Location Stem Cells and Development. Dec, 2009 | Pubmed ID: 19355838 A Single EBV-based Vector for Stable Episomal Maintenance and Expression of GFP in Human Embryonic Stem Cells Regenerative Medicine. Mar, 2009 | Pubmed ID: 19317643 Differentiating Human Multipotent Mesenchymal Stromal Cells Regulate MicroRNAs: Prediction of MicroRNA Regulation by PDGF During Osteogenesis Experimental Hematology. Oct, 2008 | Pubmed ID: 18657893 PDGF, TGF-beta, and FGF Signaling is Important for Differentiation and Growth of Mesenchymal Stem Cells (MSCs): Transcriptional Profiling Can Identify Markers and Signaling Pathways Important in Differentiation of MSCs into Adipogenic, Chondrogenic, and Osteogenic Lineages Blood. Jul, 2008 | Pubmed ID: 18332228 Concise Review: MicroRNA Expression in Multipotent Mesenchymal Stromal Cells Stem Cells (Dayton, Ohio). Feb, 2008 | Pubmed ID: 17991914 Creation of Engineered Human Embryonic Stem Cell Lines Using PhiC31 Integrase Stem Cells (Dayton, Ohio). Jan, 2008 | Pubmed ID: 17962703 Gene Transfer Via Nucleofection into Adult and Embryonic Stem Cells Methods in Molecular Biology (Clifton, N.J.). Month, 2007 | Pubmed ID: 18453252 Micro RNA Profiling: an Easy and Rapid Method to Screen and Characterize Stem Cell Populations Methods in Molecular Biology (Clifton, N.J.). Month, 2007 | Pubmed ID: 18453251 MicroRNA Expression Pattern of Undifferentiated and Differentiated Human Embryonic Stem Cells Stem Cells and Development. Dec, 2007 | Pubmed ID: 18004940 Qualification of Embryonal Carcinoma 2102Ep As a Reference for Human Embryonic Stem Cell Research Stem Cells (Dayton, Ohio). Feb, 2007 | Pubmed ID: 17284651 Stem Cell Plasticity Blood Reviews. Jan, 2005 | Pubmed ID: 15572215 Efficient Transfection of Embryonic and Adult Stem Cells Stem Cells (Dayton, Ohio). Month, 2004 | Pubmed ID: 15277699 Expression of Bacterial Endonucleases in Saccharomyces Cerevisiae Mitochondria Mitochondrion. Nov, 2002 | Pubmed ID: 16120308 Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR Suman K. Pradhan1, Jerry Guzman1, Carl Dargitz1, Stephanie Switalski2, Mark Landon1, Sven Olek3, Bjoern Samans3, Ulrich Hoffmueler3, Uma Lakshmipathy1 1Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, 2California Polytechnic State University (Cal Poly), 3Epiontis GmbH, Precision for Medicine JoVE 60465 면역학 및 감염병학 Kinetic Measurement and Real Time Visualization of Somatic Reprogramming Rene H. Quintanilla Jr.1, Joanna Asprer1, Kyle Sylakowski1, Uma Lakshmipathy1 1Life Sciences Solutions, Thermo Fisher Scientific JoVE 54190 발생학
Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCR Suman K. Pradhan1, Jerry Guzman1, Carl Dargitz1, Stephanie Switalski2, Mark Landon1, Sven Olek3, Bjoern Samans3, Ulrich Hoffmueler3, Uma Lakshmipathy1 1Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, 2California Polytechnic State University (Cal Poly), 3Epiontis GmbH, Precision for Medicine JoVE 60465 면역학 및 감염병학
Kinetic Measurement and Real Time Visualization of Somatic Reprogramming Rene H. Quintanilla Jr.1, Joanna Asprer1, Kyle Sylakowski1, Uma Lakshmipathy1 1Life Sciences Solutions, Thermo Fisher Scientific JoVE 54190 발생학