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Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation
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June 28, 2024
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This article describes a protocol for aggregating and encapsulating spleen cells within a semi-solid basement membrane matrix. Basement membrane matrix constructs can be used in three-dimensional culture for studying organoid development, or for in vivo transplantation and tissue regeneration studies.
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Tourle, K., Rucinski, A., Grainger, A., Limnios, I. J., Gonzalez Ruiz, M., Tan, J. K. Basement Membrane Matrix Encapsulated Cell Aggregation for Investigating Murine Spleen Tissue Formation. J. Vis. Exp. (208), e66682, doi:10.3791/66682 (2024).
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