Journal
/
Immunology and Infection
/
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

English

Automatically Generated

824 Views

•

06:28 min

•

February 17, 2023

DOI:

10.3791/65062-v

DOI:
10.3791/65062-v

•

06:28 min
February 17, 2023

•

4 Views
, ,
1Department of Clinical Immunology,Jagiellonian University Medical College, 2Doctoral School of Medical and Health Sciences,Jagiellonian University, 3Institute of Veterinary Sciences, University Center of Veterinary Medicine JU-AU,University of Agriculture in Kraków

Summary

Automatically generated

The proposed protocol includes guidelines on how to avoid contamination with endotoxin during the isolation of extracellular vesicles from cell culture supernatants, and how to properly evaluate them.

Explore More Videos

Extracellular VesiclesEndotoxinLow-endotoxinCell CultureSupernatantUltracentrifugationFiltrationSW480SW620PBSFBSAseptic ProcedurePyrogenicLow Protein Binding

Read Article

Cite this Article

Babula, A., Siemińska, I., Baj-Krzyworzeka, M. Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines. J. Vis. Exp. (192), e65062, doi:10.3791/65062 (2023).

Download .ris file

Copy

Share Video

.

Copy