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January 05, 2022
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Here, a method is described for making plant tissues transparent while maintaining the stability of fluorescent proteins. This technique facilitates deep imaging of cleared plant tissues without physical sectioning.
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Kurihara, D., Mizuta, Y., Nagahara, S., Sato, Y., Higashiyama, T. Optical Clearing of Plant Tissues for Fluorescence Imaging. J. Vis. Exp. (179), e63428, doi:10.3791/63428 (2022).
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