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Laser Micro-Irradiation to Study DNA Recruitment During S Phase
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Biology
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JoVE Journal Biology
Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

English

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3,811 Views

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07:11 min

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April 16, 2021

DOI:

10.3791/62466-v

DOI:
10.3791/62466-v

•

07:11 min
April 16, 2021

•

19 Views
,
1Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, 2Laura and Isaac Perlmutter Cancer Center,New York University School of Medicine, 3Howard Hughes Medical Institute,New York University School of Medicine

Summary

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This protocol describes a non-invasive method to efficiently identify S-phase cells for downstream microscopy studies, such as measuring DNA repair protein recruitment by laser micro-irradiation.

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Cite this Article

Miwatani-Minter, B., Rona, G. Laser Micro-Irradiation to Study DNA Recruitment During S Phase. J. Vis. Exp. (170), e62466, doi:10.3791/62466 (2021).

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