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Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
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Immunology and Infection
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JoVE Journal Immunology and Infection
Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

English

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5,410 Views

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06:25 min

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February 08, 2020

DOI:

10.3791/60731-v

DOI:
10.3791/60731-v

•

06:25 min
February 08, 2020

•

3 Views
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1Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences,Zhejiang University

Summary

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We describe an immunofluorescence and quantitative PCR method for the localization and quantification of begomoviruses in insect tissues. The immunofluorescence protocol can be used to colocalize viral and vector proteins. The quantitative PCR protocol can be extended to quantify viruses in whole whitefly bodies and virus-infected plants.

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Cite this Article

Ban, F., Yin, T., Guo, Q., Pan, L., Liu, Y., Wang, X. Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR. J. Vis. Exp. (156), e60731, doi:10.3791/60731 (2020).

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