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Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy
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Biochemistry
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JoVE Journal Biochemistry
Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

English

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9,479 Views

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12:58 min

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September 12, 2019

DOI:

10.3791/60108-v

DOI:
10.3791/60108-v

•

12:58 min
September 12, 2019

•

5 Views
, , , ,
1Centre for Misfolding Diseases, Department of Chemistry,University of Cambridge, 2Institute of Biotechnology, Life Sciences Center,Vilnius University, 3Cavendish Laboratory, Department of Physics,University of Cambridge

Summary

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We describe the application of infrared nanospectroscopy and high-resolution atomic force microscopy to visualize the process of protein self-assembly into oligomeric aggregates and amyloid fibrils, which is closely associated with the onset and development of a wide range of human neurodegenerative disorders.

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Cite this Article

Ruggeri, F. S., Šneideris, T., Chia, S., Vendruscolo, M., Knowles, T. P. J. Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy. J. Vis. Exp. (151), e60108, doi:10.3791/60108 (2019).

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