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Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish
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Immunology and Infection
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JoVE Journal Immunology and Infection
Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish

English

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10,285 Views

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07:00 min

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October 08, 2018

DOI:

10.3791/58299-v

DOI:
10.3791/58299-v

•

07:00 min
October 08, 2018

•

5 Views
, , ,
1Faculty of Medicine and Life Sciences,University of Tampere, 2Oral and Maxillofacial Unit,Tampere University Hospital

Summary

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Here, we present a protocol to model human tuberculosis in an adult zebrafish using its natural pathogen Mycobacterium marinum. Extracted DNA and RNA from the internal organs of infected zebrafish can be used to reveal the total mycobacterial loads in the fish and the host's immune responses with qPCR.

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Luukinen, H., Hammarén, M. M., Vanha-aho, L., Parikka, M. Modeling Tuberculosis in Mycobacterium marinum Infected Adult Zebrafish. J. Vis. Exp. (140), e58299, doi:10.3791/58299 (2018).

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