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Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
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Genetics
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JoVE Journal Genetics
Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

English

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10,183 Views

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09:57 min

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December 28, 2016

DOI:

10.3791/54976-v

DOI:
10.3791/54976-v

•

09:57 min
December 28, 2016

•

23 Views
, ,
1Entomology Department,University of Maryland, 2Program in Molecular and Cell Biology,University of Maryland

Summary

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Here, we present protocols for rearing an intermediate-germ beetle, Dermestes maculatus (D. maculatus) in the lab. We also share protocols for embryonic and parental RNAi and methods for analyzing embryonic phenotypes to study gene function in this species.

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Dermestes MaculatusHide BeetleRearingDouble-stranded RNAGene KnockdownInsect Model SystemRNA InterferenceDevelopmental BiologyEvolutionary BiologyInsect Pest Control

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Xiang, J., Reding, K., Pick, L. Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus. J. Vis. Exp. (118), e54976, doi:10.3791/54976 (2016).

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