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Analysis of Coronary Vessels in Cleared Embryonic Hearts
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Analysis of Coronary Vessels in Cleared Embryonic Hearts

Analysis of Coronary Vessels in Cleared Embryonic Hearts

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08:25 min

December 07, 2016

DOI:

08:25 min
December 07, 2016

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Summary

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We present a protocol for the analysis of coronary vessels in whole embryonic murine hearts up to E15.5, using standard immunological staining methods followed by optical clearance and confocal microscopy. This technique enables visualization of blood vessels throughout the entire heart without the need for time-consuming analysis of serial sections.

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