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Immunology and Infection
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Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling
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Immunology and Infection
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JoVE Journal Immunology and Infection
Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

English

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28,924 Views

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09:43 min

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April 16, 2015

DOI:

10.3791/52687-v

DOI:
10.3791/52687-v

•

09:43 min
April 16, 2015

•

45 Views
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1LD MacLean Surgical Research Laboratories, Department of Surgery,McGill University

Summary

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In the following protocol, we describe a very simple way to isolate Neutrophil Extracellular traps (NETs) from human whole blood using readily available reagents. We then demonstrate how the isolated NETs can be used in an in vitro adhesion assay with cancer cells.

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Cite this Article

Najmeh, S., Cools-Lartigue, J., Giannias, B., Spicer, J., Ferri, L. E. Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling. J. Vis. Exp. (98), e52687, doi:10.3791/52687 (2015).

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