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Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis
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Neuroscience
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JoVE Journal Neuroscience
Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis

Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis

English

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12,294 Views

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09:38 min

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August 30, 2013

DOI:

10.3791/50769-v

DOI:
10.3791/50769-v

•

09:38 min
August 30, 2013

•

1 Views
, , , , ,
1Department of Neurologic Surgery,Mayo Clinic College of Medicine, 2Department of Anatomy,Korea University College of Medicine, 3Department of Biochemistry and Molecular Biology,Mayo Clinic College of Medicine

Summary

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During adult hippocampal neurogenesis, a distinct set of genetic markers are expressed when a quiescent neural stem cell sequentially progresses and develops into a functionally integrated neuron in the circuit. Using heat-induced antigen retrieval, progenitor cell types that are otherwise difficult to detect are identified with improved effectiveness.

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Heat-induced Antigen RetrievalImmunohistochemistryAdult Hippocampal NeurogenesisNeural Stem CellProgenitor Cell TypesProliferationDifferentiationMaturationFunctional IntegrationPathophysiology

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Cite this Article

Hussaini, S. M., Jun, H., Cho, C. H., Kim, H. J., Kim, W. R., Jang, M. Heat-Induced Antigen Retrieval: An Effective Method to Detect and Identify Progenitor Cell Types during Adult Hippocampal Neurogenesis. J. Vis. Exp. (78), e50769, doi:10.3791/50769 (2013).

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