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Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
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September 16, 2013
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Human neuroprogenitor cells (NPCs) were expanded under proliferating conditions. NPCs were differentiated into neuron-rich cultures in the presence of a combination of neurotrophins. Neuronal markers were detected by immunofluorescence staining. To isolate a pure population of neurons, NPCs were differentiated on PEN membrane slides and laser capture microdissection was performed.
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Bouchard, R., Chong, T., Pugazhenthi, S. Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture. J. Vis. Exp. (79), e50487, doi:10.3791/50487 (2013).
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