Simultaneous Pre- and Post-synaptic Electrophysiological Recording from <em>Xenopus</em> Nerve-muscle Co-cultures

Bruce Yazejian; Rita M. Yazejian; Rachel Einarsson; Alan D. Grinnell

doi:10.3791/50253

Journal

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Neuroscience

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Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

10,731 Views

•

08:13 min

•

March 11, 2013

DOI:

10.3791/50253-v

DOI:

10.3791/50253-v

•

08:13 min

March 11, 2013

•

1 Views

Bruce Yazejian¹, Rita M. Yazejian¹, Rachel Einarsson², Alan D. Grinnell¹

¹Department of Physiology,David Geffen School of Medicine at UCLA, ²Natural Science Division,Pepperdine University

Summary

This video demonstrates the procedures used to grow primary cultures of embryonic Xenopus nerve and muscle cells and the usefulness of this preparation for making simultaneous pre- and post-synaptic patch clamp recordings.

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Simultaneous Pre- and Post-synaptic Electrophysiological Recording from Xenopus Nerve-muscle Co-cultures

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