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Murine Superficial Lymph Node Surgery
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Immunology and Infection
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JoVE Journal Immunology and Infection
Murine Superficial Lymph Node Surgery

Murine Superficial Lymph Node Surgery

English

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41,578 Views

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04:36 min

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May 21, 2012

DOI:

10.3791/3444-v

DOI:
10.3791/3444-v

•

04:36 min
May 21, 2012

•

37 Views
,
1Maisonneuve-Rosemont Hospital Research Center, 2Department of Microbiology and Immunology,University of Montreal, 3Department of Medicine,University of Montreal

Summary

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To follow the progression of an immune response over time within the same mouse, lymph nodes can be sequentially removed by surgery. Here, we describe how this technique can be performed.

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MurineSuperficial Lymph Node SurgeryImmunologyImmune ResponseVaccineInfectionTumorLymphocyte HomeostasisTime Course ExperimentsExperimental VariabilityBlood WithdrawalCirculating LymphocytesLymph NodesSurgeryImmune Cell ExpansionPhenotypic CharacterizationFunctional AssaysBrachial Lymph NodesInguinal Lymph NodesSmall Incision In The SkinAnesthetized MouseQuick RecoveryMinimal Pain

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Cite this Article

Mathieu, M., Labrecque, N. Murine Superficial Lymph Node Surgery. J. Vis. Exp. (63), e3444, doi:10.3791/3444 (2012).

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