JOVE JOURNAL

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Journal (Developmental Biology)

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Sarah Avila-Barnard¹, David C. Volz¹

¹Department of Environmental Sciences,University of California, Riverside

Journal (Developmental Biology)

Protocol for Human Blastoids Modeling Blastocyst Development and Implantation

Harunobu Kagawa*¹, Alok Javali*¹, Heidar Heidari Khoei*¹, Theresa Maria Sommer^1,3, Giovanni Sestini^1,3, Maria Novatchkova², Yvonne Scholte op Reimer¹, Nicolas Rivron¹

¹Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA),Vienna Biocenter (VBC), ²Institute of Molecular Pathology (IMP),Vienna Biocenter (VBC), ³Vienna BioCenter PhD Program,Doctoral School of the University of Vienna and Medical University of Vienna

Journal (Developmental Biology)

Isolation of Preadipocytes from Broiler Chick Embryos

Minjeong Kim¹, Usuk Jung¹, Elizabeth Shepherd¹, Robert Mihelic¹, Brynn H. Voy¹

¹Department of Animal Science,University of Tennessee

Journal (Developmental Biology)

Fluorescent In Situ Hybridization and 5-Ethynyl-2′-Deoxyuridine Labeling for Stem-Like Cells in the Hydrozoan Jellyfish Cladonema pacificum

Sosuke Fujita¹, Erina Kuranaga¹, Masayuki Miura², Yu-ichiro Nakajima²

¹Graduate School of Life Sciences,Tohoku University, ²Graduate School of Pharmaceutical Sciences,The University of Tokyo

Journal (Developmental Biology)

Generation of a Mouse Artificial Decidualization Model with Ovariectomy for Endometrial Decidualization Research

Yang Zhang*^1,2,3, Zhe Zhang*^1,2,3, Nannan Kang^1,2,3, Xiaoqiang Sheng^1,2,3

¹Center for Reproductive Medicine and Obstetrics and Gynecology,The Affiliated Drum Tower Hospital of Nanjing University Medical School, ²State Key Laboratory of Reproductive Medicine,Nanjing Medical University, ³Center for Molecular Reproductive Medicine,Nanjing University

Journal (Developmental Biology)

En Face Endocardial Cushion Preparation for Planar Morphogenesis Analysis in Mouse Embryos

Tamara Gonzalez-Costa^1,2, Jose Luis de la Pompa^1,2, Joaquim Grego-Bessa^1,2

¹Intercellular Signalling in Cardiovascular Development and Disease Laboratory,Centro Nacional de Investigaciones Cardiovasculares (CNIC), ²Ciber de Enfermedades Cardiovasculares

Journal (Developmental Biology)

Live Imaging of Early Cardiac Progenitors in the Mouse Embryo

Miquel Sendra¹, Jorge Mañes¹, Jorge N. Domínguez^2,3, Miguel Torres¹

¹Cardiovascular Regeneration Program,Centro Nacional de Investigaciones Cardiovasculares (CNIC), ²Cardiovascular Development Group, Department of Experimental Biology,University of Jaén, Jaén, ³Fundación Medina, Granada

Journal (Developmental Biology)

Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte

Laura Adams*¹, Ying Liu*¹, Barbara Durrant², Elena Ruggeri², Carly Young², Rebecca Krisher³, Irina Polejaeva¹

¹Department of Animal, Dairy, and Veterinary Sciences,Utah State University, ²Reproductive Sciences, Beckman Center for Conservation Research,San Diego Zoo Wildlife Alliance, ³Genus PLC

Journal (Developmental Biology)

Polysome Purification from Soybean Symbiotic Nodules

María Martha Sainz¹, Carla Valeria Filippi¹, Guillermo Eastman^2,3, Mariana Sotelo-Silveira¹, C. Mauro Martinez¹, Omar Borsani¹, José Sotelo-Silveira^2,4

¹Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía,Universidad de la República, ²Departamento de Genómica,Instituto de Investigaciones Biológicas Clemente Estable, ³Department of Biology,University of Virginia, ⁴Departamento de Biología Celular y Molecular, Facultad de Ciencias,Universidad de la República

Journal (Developmental Biology)

Preparation and Morphological Analysis of Chick Cranial Neural Crest Cell Cultures

Bridget T. Jacques-Fricke¹, Julaine Roffers-Agarwal^2,3, Callie M. Gustafson^2,3, Laura S. Gammill^2,3

¹Department of Biology and Neuroscience Program,Hamline University, ²Department of Genetics, Cell Biology and Development,University of Minnesota, ³Developmental Biology Center,University of Minnesota

Journal (Developmental Biology)

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

Yoko Tanimoto*¹, Natsuki Mikami*^1,2, Miyuki Ishida¹, Natsumi Iki¹, Kanako Kato¹, Fumihiro Sugiyama¹, Satoru Takahashi¹, Seiya Mizuno¹

¹Laboratory Animal Resource Center and Trans-Border Medical Research Center,University of Tsukuba, ²Ph.D. Program in Human Biology, School of Integrative and Global Majors,University of Tsukuba

Journal (Developmental Biology)

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development

Nishant Singh*^1,2, Anubhav Prakash*¹, Suraj Ranganath Chakravarthy*¹, Raman Kaushik*¹, Raj K. Ladher¹

¹National Centre for Biological Sciences,Tata Institute of Fundamental Research, ²The University of Trans-Disciplinary Health Sciences and Technology

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