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The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble
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生化学
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JoVE Journal 生化学
The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

The Automated Crystallography Pipelines at the EMBL HTX Facility in Grenoble

DOI:
10.3791/62491-v

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06:50 min

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June 05, 2021

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, , , , , , , , , ,
1European Molecular Biology Laboratory, 2ALPX S.A.S., 3Institut de Biologie Structurale

章

  • 00:04Introduction
  • 00:32Crystallization Screening Experiments
  • 02:42Crystal Optimization Experiments
  • 03:18Crystal Harvesting and Tracking
  • 04:21Synchrotron X-Ray Diffraction Experiments
  • 05:27結果
  • 06:08Conclusion

概要

自動翻訳

自動翻訳

Here, we describe how to use the automated macromolecular crystallography pipelines for protein-to-structure, rapid ligand-protein complex analysis and large-scale fragment screening based on the CrystalDirect technology at the HTX Laboratory in EMBL Grenoble.

タグ

Automated CrystallographyEMBL HTX FacilityGrenobleCrystalDirect TechnologyCRIM SoftwareCrystal Graphic Information Management SystemCrystallization ProtocolCrystallization ScreensHigh Throughput Crystallization LabAutomated ImagersCRIMSCrystallization PlatesCrystallization ExperimentsCrystal HarvestingProtein Structure DeterminationFragment ScreeningLigand Screening

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