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ケラチノサイト幹細胞の可塑性をモデル化する高スループット表皮球面培養システムの確立
1Department of Pathology, Microbiology and Immunology,University of South Carolina School of Medicine, 2皮膚学部,Brigham and Women’s Hospital, Harvard Medical School, 3生化学・分子生物学科,University of Maryland School of Medicine Baltimore, 4病理学科,Albert Einstein College of Medicine, 5小児科,Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy,University of South Carolina
章
- 00:04Introduction
- 00:27Isolation and Culture of Human Keratinocytes from Neonatal Foreskin Tissue
- 02:37Generating Skin Epidermosphere Cultures in Vitro
- 05:02Epidermal Spheroid Re-Plating Assay
- 05:42Characterization of Spheroid-Derived (SD) Sub-Populations by FACS
- 08:06Results: Characterization of Epidermal Spheroid Formation and Spheroid-Derived NHKs
- 09:30Conclusion
