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Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
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JoVE Journal 発生生物学
Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity

DOI:
10.3791/62182-v

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10:03 min

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January 30, 2021

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, , , , ,
1Department of Pathology, Microbiology and Immunology,University of South Carolina School of Medicine, 2皮膚学部,Brigham and Women’s Hospital, Harvard Medical School, 3生化学・分子生物学科,University of Maryland School of Medicine Baltimore, 4病理学科,Albert Einstein College of Medicine, 5小児科,Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy,University of South Carolina

章

  • 00:04Introduction
  • 00:27Isolation and Culture of Human Keratinocytes from Neonatal Foreskin Tissue
  • 02:37Generating Skin Epidermosphere Cultures in Vitro
  • 05:02Epidermal Spheroid Re-Plating Assay
  • 05:42Characterization of Spheroid-Derived (SD) Sub-Populations by FACS
  • 08:06Results: Characterization of Epidermal Spheroid Formation and Spheroid-Derived NHKs
  • 09:30Conclusion

概要

自動翻訳

自動翻訳

Here we describe a protocol for the systematic cultivation of epidermal spheroids in 3D suspension culture. This protocol has wide-ranging applications for use in a variety of epithelial tissue types and for the modeling of several human diseases and conditions.

タグ

Epidermal SpheroidKeratinocyte Stem CellHigh Throughput CultureNeonatal ForeskinDispaseTrypsin-EDTAKSFM-SCM MediumAgarose96-well Plate

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