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Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity
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January 30, 2021
DOI:
1Department of Pathology, Microbiology and Immunology,University of South Carolina School of Medicine, 2皮膚学部,Brigham and Women’s Hospital, Harvard Medical School, 3生化学・分子生物学科,University of Maryland School of Medicine Baltimore, 4病理学科,Albert Einstein College of Medicine, 5小児科,Vanderbilt University Medical Center, 6Department of Drug Discovery and Biomedical Sciences, College of Pharmacy,University of South Carolina
概要
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Here we describe a protocol for the systematic cultivation of epidermal spheroids in 3D suspension culture. This protocol has wide-ranging applications for use in a variety of epithelial tissue types and for the modeling of several human diseases and conditions.
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Woappi, Y., Ezeka, G., Vercellino, J., Bloos, S. M., Creek, K. E., Pirisi, L. Establishing a High Throughput Epidermal Spheroid Culture System to Model Keratinocyte Stem Cell Plasticity. J. Vis. Exp. (167), e62182, doi:10.3791/62182 (2021).
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