ジャーナル
/
神経科学
/
Analysis of Translation in the Developing Mouse Brain using Polysome Profiling
JoVE Journal
神経科学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 神経科学
Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

4,556 Views

•

08:38 min

•

May 22, 2021

DOI:

10.3791/62088-v

DOI:
10.3791/62088-v

•

08:38 min
May 22, 2021

•

8 Views
, ,
1Department of Biochemistry and Molecular Biology; Cumming School of Medicine,University of Calgary, 2Department of Medical Genetics, Cumming School of Medicine,University of Calgary, 3Alberta Children’s Hospital Research Institute,University of Calgary

概要

Automatically generated

The development of the mammalian brain requires proper control of gene expression at the level of translation. Here, we describe a polysome profiling system with an easy-to-assemble sucrose gradient-making and fractionation platform to assess the translational status of mRNAs in the developing brain.

Explore More Videos

Polysome ProfilingTranslational RegulationBrain DevelopmentSucrose GradientEmbryonic Day 12Mouse CortexCycloheximideRNA Extraction

Read Article

Cite this Article

Kedia, S., Erickson, S. L., Yang, G. Analysis of Translation in the Developing Mouse Brain using Polysome Profiling. J. Vis. Exp. (171), e62088, doi:10.3791/62088 (2021).

Download .ris file

Copy

Share Video

.

Copy