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Analysis of Translation in the Developing Mouse Brain using Polysome Profiling
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JoVE Journal Neuroscienze
Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

DOI:
10.3791/62088-v

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08:38 min

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May 22, 2021

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, ,
1Department of Biochemistry and Molecular Biology; Cumming School of Medicine,University of Calgary, 2Department of Medical Genetics, Cumming School of Medicine,University of Calgary, 3Alberta Children’s Hospital Research Institute,University of Calgary

Capitoli

  • 00:04Introduction
  • 00:50Preparation of Sucrose Gradient
  • 01:57Tissue Dissection
  • 03:05Cell Lysis
  • 04:34Fractionation and Sample Collection
  • 06:15Results: Polysome Profiling Analysis of the Developing Mouse Cortex
  • 07:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

The development of the mammalian brain requires proper control of gene expression at the level of translation. Here, we describe a polysome profiling system with an easy-to-assemble sucrose gradient-making and fractionation platform to assess the translational status of mRNAs in the developing brain.

Tags

Polysome ProfilingTranslational RegulationBrain DevelopmentSucrose GradientEmbryonic Day 12Mouse CortexCycloheximideRNA Extraction

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