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Analysis of Translation in the Developing Mouse Brain using Polysome Profiling
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神经科学
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JoVE 杂志 神经科学
Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

4,556 Views

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08:38 min

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May 22, 2021

DOI:

10.3791/62088-v

DOI:
10.3791/62088-v

•

08:38 min
May 22, 2021

•

8 Views
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1Department of Biochemistry and Molecular Biology; Cumming School of Medicine,University of Calgary, 2Department of Medical Genetics, Cumming School of Medicine,University of Calgary, 3Alberta Children’s Hospital Research Institute,University of Calgary

Summary

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The development of the mammalian brain requires proper control of gene expression at the level of translation. Here, we describe a polysome profiling system with an easy-to-assemble sucrose gradient-making and fractionation platform to assess the translational status of mRNAs in the developing brain.

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Cite this Article

Kedia, S., Erickson, S. L., Yang, G. Analysis of Translation in the Developing Mouse Brain using Polysome Profiling. J. Vis. Exp. (171), e62088, doi:10.3791/62088 (2021).

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