This protocol describes how to perform optogenetic experiments for controlling gene expression with red and far-red light using PhyB and PIF3. Included are step-by-step instructions for building a simple and flexible illumination system, which enables the control of gene expression or other optogenetics with a computer.
Kyriakakis, P., Fernandez de Cossio, L., Howard, P. W., Kouv, S., Catanho, M., Hu, V. J., Kyriakakis, R., Allen, M. E., Ma, Y., Aguilar-Rivera, M., Coleman, T. P. Building a Simple and Versatile Illumination System for Optogenetic Experiments. J. Vis. Exp. (167), e61914, doi:10.3791/61914 (2021).