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从多个中枢神经系统区域解剖和分离小鼠神经胶质细胞
JoVE Journal
神経科学
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JoVE Journal 神経科学
Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions
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从多个中枢神经系统区域解剖和分离小鼠神经胶质细胞

2,842 Views

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08:00 min

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June 04, 2020

DOI:

10.3791/61345-v

DOI:
10.3791/61345-v

•

08:00 min
June 04, 2020

•

2 Views
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1Department of Neurosciences,Lerner Research Institute, Cleveland Clinic Foundation, 2Brain Health Research Institute,Kent State University

概要

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在这里，我们提出了一种从小鼠CNS体外分离多个神经胶质细胞群的方案。该方法允许分离区域小胶质细胞、少突胶质细胞前体细胞和星形胶质细胞，以研究每种细胞在各种培养系统中的表型。

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Cite this Article

Sinyuk, M., Williams, J. L. Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions. J. Vis. Exp. (160), e61345, doi:10.3791/61345 (2020).

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