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Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions

Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions

2,869 Views

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08:00 min

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June 04, 2020

DOI:

10.3791/61345-v

DOI:
10.3791/61345-v

•

08:00 min
June 04, 2020

•

2 Views
,
1Department of Neurosciences,Lerner Research Institute, Cleveland Clinic Foundation, 2Brain Health Research Institute,Kent State University

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Here we present a protocol for in vitro isolation of multiple glial cell populations from a mouse CNS. This method allows for the segregation of regional microglia, oligodendrocyte precursor cells, and astrocytes to study the phenotypes of each in a variety of culture systems.

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Cite this Article

Sinyuk, M., Williams, J. L. Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions. J. Vis. Exp. (160), e61345, doi:10.3791/61345 (2020).

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