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Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions
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Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions
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June 04, 2020
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Here we present a protocol for in vitro isolation of multiple glial cell populations from a mouse CNS. This method allows for the segregation of regional microglia, oligodendrocyte precursor cells, and astrocytes to study the phenotypes of each in a variety of culture systems.
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Sinyuk, M., Williams, J. L. Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions. J. Vis. Exp. (160), e61345, doi:10.3791/61345 (2020).
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