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神经科学
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Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions
JoVE 杂志
神经科学
This content is Free Access.
JoVE 杂志 神经科学
Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions

Dissection and Isolation of Murine Glia from Multiple Central Nervous System Regions

DOI:
10.3791/61345-v

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08:00 min

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June 04, 2020

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1Department of Neurosciences,Lerner Research Institute, Cleveland Clinic Foundation, 2Brain Health Research Institute,Kent State University

Chapters

  • 00:05Introduction
  • 00:38Cortex, Cerebellum, Brainstem, and Spinal Cord Dissection
  • 02:51Tissue Dissociation and Oligodendrocyte Precursor Cell Isolation
  • 06:00Results: OPC Differentiation in IFNγR-/- Mice
  • 07:31Conclusion

Summary

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自动翻译

Here we present a protocol for in vitro isolation of multiple glial cell populations from a mouse CNS. This method allows for the segregation of regional microglia, oligodendrocyte precursor cells, and astrocytes to study the phenotypes of each in a variety of culture systems.

Tags

Murine GliaCentral Nervous SystemGlia HeterogeneityGlia IsolationTrypsinDNase ICell SuspensionRegional Differences

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