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使用 iDISCO + 和光片荧光显微镜对脊椎淋巴血管和+排水进行三维成像
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy
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使用 iDISCO + 和光片荧光显微镜对脊椎淋巴血管和+排水进行三维成像

9,590 Views

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10:05 min

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May 22, 2020

DOI:

10.3791/61099-v

DOI:
10.3791/61099-v

•

10:05 min
May 22, 2020

•

20 Views
, ,
1Université Pierre et Marie Curie Paris, Sorbonne Université,Institut du Cerveau et de la Moelle Epinière, 2Instituto de Ciências Biomédicas,Universidade Federal do Rio de Janeiro, 3Department of Neurology,Yale University School of Medicine

概要

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提出了一种将组织清除与光片荧光显微镜 （LSFM） 相结合的协议，以获得收集脑脊液 （CSF） 和脊柱硬膜外液的淋巴血管和淋巴结 （LNs） 的三维和细胞分辨率图像。

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Cite this Article

Jacob, L., Brito, J., Thomas, J. Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy. J. Vis. Exp. (159), e61099, doi:10.3791/61099 (2020).

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