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Identificatie van Neutrophil Extracellular Traps in Paraffine-Embedded Feline Arterial Thrombi using Immunofluorescentie Microscopie
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免疫学と感染
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JoVE Journal 免疫学と感染
Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy

Identificatie van Neutrophil Extracellular Traps in Paraffine-Embedded Feline Arterial Thrombi using Immunofluorescentie Microscopie

DOI:

08:48 min

March 29, 2020

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  • 00:04Introduction
  • 01:15Deparaffinization, Rehydration, and Heat-Induced Antigen Retrieval
  • 02:29Immunolabeling and Autofluorescence Quenching
  • 05:13Neutrophil Extracellular Trap Identification
  • 06:08Results: Phase Contrast and Immunofluorescence Images
  • 07:20Conclusion

概要

自動翻訳

We beschrijven een methode om neutrofiele extracellulaire vallen (NET's) te identificeren in formaldehyde-vaste en paraffine-ingebedde katachtige cardiogene trombi met behulp van warmte-geïnduceerde antigen retrieval en een dubbele immunolabeling protocol.

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