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Fluorescerende calciumbeeldvorming en daaropvolgende in situ hybridisatie voor neuronale precursorkarakterisering in Xenopus laevis
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
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Fluorescerende calciumbeeldvorming en daaropvolgende in situ hybridisatie voor neuronale precursorkarakterisering in Xenopus laevis

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00:09 min

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February 18, 2020

DOI:

10.3791/60726-v

DOI:
10.3791/60726-v

•

00:09 min
February 18, 2020

•

3 Views
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1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,Harvard University, 2生物学科,College of William and Mary, 3Harvard Medical School,Harvard University, 4生物工学科,California Institute of Technology, 5Department of Computer Science,College of William and Mary

概要

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We presenteren een tweedelige protocol dat fluorescerende calciumbeeldvorming combineert met in situ hybridisatie, waardoor de experimentator patronen van calciumactiviteit kan correleren met genexpressieprofielen op een eencellend niveau.

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Ablondi, E. F., Paudel, S., Sehdev, M., Marken, J. P., Halleran, A. D., Rahman, A., Kemper, P., Saha, M. S. Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis. J. Vis. Exp. (156), e60726, doi:10.3791/60726 (2020).

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