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Imaging fluorescente del calcio e successiva ibridazione in situ per la caratterizzazione neuronale del precursore in Xenopus laevis
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis
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Imaging fluorescente del calcio e successiva ibridazione in situ per la caratterizzazione neuronale del precursore in Xenopus laevis

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00:09 min

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February 18, 2020

DOI:

10.3791/60726-v

DOI:
10.3791/60726-v

•

00:09 min
February 18, 2020

•

3 Views
, , , , , , ,
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School,Harvard University, 2生物学科,College of William and Mary, 3Harvard Medical School,Harvard University, 4生物工学科,California Institute of Technology, 5Department of Computer Science,College of William and Mary

概要

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Vi presentiamo un protocollo in due parti che combina l'imaging fluorescente del calcio con l'ibridazione in situ, permettendo allo sperimentatore di correlare i modelli di attività del calcio con i profili di espressione genica a livello unicellulare.

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Cite this Article

Ablondi, E. F., Paudel, S., Sehdev, M., Marken, J. P., Halleran, A. D., Rahman, A., Kemper, P., Saha, M. S. Fluorescent Calcium Imaging and Subsequent In Situ Hybridization for Neuronal Precursor Characterization in Xenopus laevis. J. Vis. Exp. (156), e60726, doi:10.3791/60726 (2020).

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