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Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
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JoVE Journal 生化学
Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

6,170 Views

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10:27 min

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July 08, 2019

DOI:

10.3791/59918-v

DOI:
10.3791/59918-v

•

10:27 min
July 08, 2019

•

1 Views
, ,
1化学科,University of Houston, 2Department of Biology and Biochemistry,University of Houston

概要

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Dual DNA ruler assay is developed to determine the mRNA position during ribosome translocation, which relies on the dissociation forces of the formed DNA-mRNA duplexes. With single-nucleotide resolution and capability of reaching both ends of mRNA, it can provide mechanistic insights for ribosome translocation and probe other nucleic acid displacements.

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Cite this Article

Yin, H., Xu, S., Wang, Y. Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution. J. Vis. Exp. (149), e59918, doi:10.3791/59918 (2019).

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