Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
6,170 Views
•
10:27 min
•
July 08, 2019
DOI:
Summary
Automatically generated
Dual DNA ruler assay is developed to determine the mRNA position during ribosome translocation, which relies on the dissociation forces of the formed DNA-mRNA duplexes. With single-nucleotide resolution and capability of reaching both ends of mRNA, it can provide mechanistic insights for ribosome translocation and probe other nucleic acid displacements.
Read Article
Cite this Article
Yin, H., Xu, S., Wang, Y. Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution. J. Vis. Exp. (149), e59918, doi:10.3791/59918 (2019).
Copy