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Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
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生物化学
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JoVE 杂志 生物化学
Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

DOI:
10.3791/59918-v

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10:27 min

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July 08, 2019

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, ,
1Department of Chemistry,University of Houston, 2Department of Biology and Biochemistry,University of Houston

Chapters

  • 00:04Title
  • 00:43Sample Preparation
  • 04:23Magnetic and Force Measurements
  • 08:04Results: Dual DNA Ruler Assay and Probing Translocation
  • 09:51Conclusion

Summary

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Dual DNA ruler assay is developed to determine the mRNA position during ribosome translocation, which relies on the dissociation forces of the formed DNA-mRNA duplexes. With single-nucleotide resolution and capability of reaching both ends of mRNA, it can provide mechanistic insights for ribosome translocation and probe other nucleic acid displacements.

Tags

Dual DNA RulerRibosome TranslocationSingle-nucleotide ResolutionDNA BindingChemotherapeutical DrugsStreptavidinRibosome ComplexAntibioticsNeomycinEFGGTPPEPPyruvate KinaseFusidic AcidViomycinHygromycin BFrame ShiftingSlippery MotifU6A

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