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Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
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July 08, 2019
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Dual DNA ruler assay is developed to determine the mRNA position during ribosome translocation, which relies on the dissociation forces of the formed DNA-mRNA duplexes. With single-nucleotide resolution and capability of reaching both ends of mRNA, it can provide mechanistic insights for ribosome translocation and probe other nucleic acid displacements.
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Yin, H., Xu, S., Wang, Y. Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution. J. Vis. Exp. (149), e59918, doi:10.3791/59918 (2019).
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