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Isolation of Atrial Myocytes from Adult Mice
JoVE Journal
医学
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JoVE Journal 医学
Isolation of Atrial Myocytes from Adult Mice

Isolation of Atrial Myocytes from Adult Mice

9,712 Views

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08:34 min

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July 25, 2019

DOI:

10.3791/59588-v

DOI:
10.3791/59588-v

•

08:34 min
July 25, 2019

•

39 Views
,
1Libin Cardiovascular Institute of Alberta, Department of Cardiac Sciences, Department of Physiology and Pharmacology, Cumming School of Medicine,University of Calgary

概要

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This protocol is used to isolate single atrial cardiomyocytes from the adult mouse heart using a chunk digestion approach. This approach is used to isolate right or left atrial myocytes that can be used to characterize atrial myocyte electrophysiology in patch-clamp studies.

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Cite this Article

Jansen, H. J., Rose, R. A. Isolation of Atrial Myocytes from Adult Mice. J. Vis. Exp. (149), e59588, doi:10.3791/59588 (2019).

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