Journal
/
의학
/
Isolation of Atrial Myocytes from Adult Mice
JoVE 신문
의학
This content is Free Access.
JoVE 신문 의학
Isolation of Atrial Myocytes from Adult Mice

Isolation of Atrial Myocytes from Adult Mice

DOI:
10.3791/59588-v

•

08:34 min

•

July 25, 2019

•

,
1Libin Cardiovascular Institute of Alberta, Department of Cardiac Sciences, Department of Physiology and Pharmacology, Cumming School of Medicine,University of Calgary

Chapters

  • 00:04Title
  • 00:42Dissection of Mouse Atrial Appendage(s)
  • 02:46Isolation of Atrial Myocytes
  • 06:19Results: Representative Patch-clamp Data Obtained From Isolated Mouse Atrial Myocytes
  • 07:52Conclusion

Summary

자동 번역

자동 번역

This protocol is used to isolate single atrial cardiomyocytes from the adult mouse heart using a chunk digestion approach. This approach is used to isolate right or left atrial myocytes that can be used to characterize atrial myocyte electrophysiology in patch-clamp studies.

Tags

Keywords: Atrial MyocytesPatch-clampAtrial ElectrophysiologyArrhythmogenesisTrunk DigestionMouse EuthanasiaAtrial AppendageModified Tyrode’s Solution

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/59588?article_type=v

The WATCHMAN Left Atrial Appendage Closure Device for Atrial Fibrillation
check_url/kr/59588?article_type=v

Catheter Ablation in Combination With Left Atrial Appendage Closure for Atrial Fibrillation
check_url/kr/59588?article_type=v

Implanting Glass Spinal Cord Windows in Adult Mice with Experimental Autoimmune Encephalomyelitis
check_url/kr/59588?article_type=v

Robotic Ablation of Atrial Fibrillation
check_url/kr/59588?article_type=v

Isolation and Cultivation of Adult Rat Cardiomyocytes
check_url/kr/59588?article_type=v

Isolation of Atrial Cardiomyocytes from a Rat Model of Metabolic Syndrome-related Heart Failure with Preserved Ejection Fraction
check_url/kr/59588?article_type=v

Ultrasonic-augmented Primary Adult Fibroblast Isolation
check_url/kr/59588?article_type=v

Cooling or Warming the Esophagus to Reduce Esophageal Injury During Left Atrial Ablation in the Treatment of Atrial Fibrillation
check_url/kr/59588?article_type=v

Echocardiographic Evaluation of Atrial Communications before Transcatheter Closure
check_url/kr/59588?article_type=v

Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice

Read Article