Isolation of Atrial Myocytes from Adult Mice

Journal

Médecine

Journal JoVE
Médecine

This content is Free Access.

Journal JoVE Médecine

Isolation of Atrial Myocytes from Adult Mice

DOI:

10.3791/59588-v

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08:34 min

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July 25, 2019

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Hailey J. Jansen, Robert A. Rose

¹Libin Cardiovascular Institute of Alberta, Department of Cardiac Sciences, Department of Physiology and Pharmacology, Cumming School of Medicine,University of Calgary

Chapitres

00:04Titre
00:42Dissection of Mouse Atrial Appendage(s)
02:46Isolation of Atrial Myocytes
06:19Results: Representative Patch-clamp Data Obtained From Isolated Mouse Atrial Myocytes
07:52Conclusion

Summary

Traduction automatique

This protocol is used to isolate single atrial cardiomyocytes from the adult mouse heart using a chunk digestion approach. This approach is used to isolate right or left atrial myocytes that can be used to characterize atrial myocyte electrophysiology in patch-clamp studies.

Isolation of Atrial Myocytes from Adult Mice

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Chapitres

Summary

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