ジャーナル
/
/
实现干扰反射显微镜,实现无标签、高速微管成像
JoVE Journal
生物工学
This content is Free Access.
JoVE Journal 生物工学
Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
DOI:

09:45 min

August 08, 2019

,

  • 00:04標題
  • 00:46Microscope Modification and Chamber Preparation
  • 02:32Microscope Alignment
  • 03:43Control: Imaging Stabilized Microtubules
  • 04:43Imaging Microtubule Dynamics
  • 06:28Properly Setting the Aperture Diaphragm Size
  • 08:11Results: IRM Imaging of Microtubules Dynamics and Contrast Enhancement using Image Processing
  • 09:02Conclusion

概要

自動翻訳

本协议是使用体外表面测定在标准荧光显微镜上实施干扰反射显微镜的指南,用于使用体外表面测定对微管进行无标签、高对比度、高速成像。

関連ビデオ

Read Article