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Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

9,537 Views

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09:45 min

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August 08, 2019

DOI:

10.3791/59520-v

DOI:
10.3791/59520-v

•

09:45 min
August 08, 2019

•

14 Views
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1Department of Molecular Biophysics and Biochemistry,Yale University, 2Harvard Medical School,Harvard University

Summary

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This protocol is a guide for implementing interference reflection microscopy on a standard fluorescence microscope for label-free, high-contrast, high-speed imaging of microtubules using in vitro surfaces assays.

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Cite this Article

Mahamdeh, M., Howard, J. Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules. J. Vis. Exp. (150), e59520, doi:10.3791/59520 (2019).

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