This protocol is a guide for implementing interference reflection microscopy on a standard fluorescence microscope for label-free, high-contrast, high-speed imaging of microtubules using in vitro surfaces assays.
Mahamdeh, M., Howard, J. Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules. J. Vis. Exp. (150), e59520, doi:10.3791/59520 (2019).